Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Electroacupuncture Could Regulate the NF- κ B Signaling Pathway to Ameliorate the Inflammatory Injury in Focal Cerebral Ischemia/Reperfusion Model Rats

doi: 10.1155/2013/924541

Figure Lengend Snippet: Primer sequences and annealing temperatures employed for Q-PCR.

Article Snippet: The primary antibodies were NF- κ B p65 (C22B4) rabbit mAb (Cell Signaling Technology, 1 : 50), I κ B α (L35A5) mouse mAb (Cell Signaling Technology, 1 : 50), IKK α (M-110) (Santa Cruz, no. sc-7183, 1 : 25), and IKK β (P-20) (Santa Cruz, no. sc-34673, 1 : 25).

Techniques: Sequencing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Electroacupuncture Could Regulate the NF- κ B Signaling Pathway to Ameliorate the Inflammatory Injury in Focal Cerebral Ischemia/Reperfusion Model Rats

doi: 10.1155/2013/924541

Figure Lengend Snippet: Double-immunofluorescent labeling the IKK α (green) and IKK β (red) protein in sham group, I/R group, EA group, and NBD group (with the same samples from ). It showed that IKK α and IKK β were strongly expressed in cytoplasm in the I/R group. The expression of IKK β was lowly expressed in EA group and NBD group compared with the I/R group, especially in the NBD group.

Article Snippet: The primary antibodies were NF- κ B p65 (C22B4) rabbit mAb (Cell Signaling Technology, 1 : 50), I κ B α (L35A5) mouse mAb (Cell Signaling Technology, 1 : 50), IKK α (M-110) (Santa Cruz, no. sc-7183, 1 : 25), and IKK β (P-20) (Santa Cruz, no. sc-34673, 1 : 25).

Techniques: Labeling, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Electroacupuncture Could Regulate the NF- κ B Signaling Pathway to Ameliorate the Inflammatory Injury in Focal Cerebral Ischemia/Reperfusion Model Rats

doi: 10.1155/2013/924541

Figure Lengend Snippet: Fluorescent Quantitative-PCR analysis of the IKK α and IKK β mRNA expression of the sham group, I/R group, EA group, and NBD group in the ischemic brain tissue (with the same samples from ). (a) The expression of IKK α mRNA was significantly increased in the I/R group compared with that in the EA and NBD group, * P < 0.05. The IKK α mRNA expressed lowly in EA group, # P < 0.05 versus I/R group and NBD group. (b) The IKK β mRNA expression in I/R group was high at 24 h and 48 h, * P < 0.05 versus EA group and NBD group. It was lower in NBD group from 6 h to 48 h, † P < 0.05 versus EA group.

Article Snippet: The primary antibodies were NF- κ B p65 (C22B4) rabbit mAb (Cell Signaling Technology, 1 : 50), I κ B α (L35A5) mouse mAb (Cell Signaling Technology, 1 : 50), IKK α (M-110) (Santa Cruz, no. sc-7183, 1 : 25), and IKK β (P-20) (Santa Cruz, no. sc-34673, 1 : 25).

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Electroacupuncture Could Regulate the NF- κ B Signaling Pathway to Ameliorate the Inflammatory Injury in Focal Cerebral Ischemia/Reperfusion Model Rats

doi: 10.1155/2013/924541

Figure Lengend Snippet: Western blot analysis of the IKK α and IKK β protein expression in the sham group, I/R group, EA group, and NBD group in ischemic brain tissue (with the same samples from ). (a)-(b) Representative Western blot images showing bands of IKK α and IKK β proteins at 6 h, 12 h, 24 h, and 48 h. Comparison of the mean intensity ratio of immunoblotting in these groups at each timepoint. (a), (b), (c) The IKK α protein in I/R group was highly expressed, * P < 0.05 versus EA and NBD group. The expression in EA group was lower, # P < 0.05 versus NBD group. (a), (b), (d) The IKK β protein was highly expressed at 24 h and 48 h, * P < 0.05 versus EA and NBD group. The IKK β protein scarcely expressed in NBD group, † P < 0.05 versus EA group.

Article Snippet: The primary antibodies were NF- κ B p65 (C22B4) rabbit mAb (Cell Signaling Technology, 1 : 50), I κ B α (L35A5) mouse mAb (Cell Signaling Technology, 1 : 50), IKK α (M-110) (Santa Cruz, no. sc-7183, 1 : 25), and IKK β (P-20) (Santa Cruz, no. sc-34673, 1 : 25).

Techniques: Western Blot, Expressing, Comparison